Mouse embryonic stem cells differentiate into structures that resemble the neural tube and show the typical embryonic patterning
The confocal cross-section of a three-dimensional cyst shows the floor plate (green), which was formed after the addition of retinoic acid. Furthermore, the development of motor neurons (white) as well as interneurons (red) was shown. The Dresden researchers could demonstrate in vitro the typical pattern of an embryonic spinal cord.
Credit: ©CRTD/Andrea Meinhardt
Andrea Meinhardt1, Dominic Eberle1, Akira Tazaki1, Adrian Ranga4, Marco Niesche2, Michaela Wilsch-Bräuninger3, Agnieszka Stec1, Gabriele Schackert2, Matthias Lutlof4, Elly M. Tanaka1,3 3D reconstitution of the patterned neural tube from embryonic stem cells. Stem Cell Reports
1 DFG-Research Center for Regenerative Therapies Dresden – Cluster of Excellence, TU Dresden, Germany
2Faculty of Medicine, Neurosurgery Department, TU Dresden, Germany
3Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
4Laboratory of Stem Cell Bioengineering, Institute of Bioengineering, Ecole Fédérale de Lausanne, Switzerland