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FSU research uncovers new pathways for diabetes research

A new study is changing how researchers look at and the drugs used to treat the disease.

In the , Associate Professor of Chemistry and post-doctoral researcher report that a key enzyme involved in the body’s response to glucose can essentially be corrupted by a new mechanism that scientists have not seen before. This discovery shifts the current understanding of how this enzyme participates in certain diseases, including hyperinsulinemia and diabetes.

“In principle, our findings could open the door for a new approach to treat diseases of glucose homeostasis, such as diabetes,” Miller said.

The enzyme is called and serves as the body’s primary glucose sensor as it relays signals in the pancreas and liver telling other areas of the body to take up and process glucose. When that enzyme function is impaired, a person often suffers from diabetes, or other glucose-related illnesses.

Because of its role in the cell, glucokinase has received considerable attention by pharmaceutical companies as a potential therapeutic target. More than a decade ago, researchers uncovered drugs that activate the enzyme, which offered hope for treating type 2 diabetes.

The focus, however, was on only one way that the enzyme was activated. Miller and Whittington’s research showed there is actually another avenue for activation.

The work showed that this new activation mechanism is operational in clinically characterized disease forms of the enzyme. Activation of glucokinase is a known cause of hyperinsulinemia, a disease characterized by over secretion of insulin even in the absence of glucose, often leading to insulin resistance.

“Glucokinase-associated hyperinsulinemia varies in severity depending upon the level of activation of the enzyme,” Whittington said. “Our study is important because it sheds new light onto how the functional properties of the enzyme can manifest in disease.”

In addition to the direct medical implications, the new study also shifts a fundamental principle of biochemistry known as allostery. Allostery is one way in which the function of proteins in the cell are controlled and regulated. Previously, scientists believed that allosteric regulation of glucokinase depended on strict structural transitions of the biomolecule. But, the new research indicates, that regulation of the enzyme relies not only on its structure, but also on its inherent flexibility and how the enzyme moves over different periods of time.

In collaboration with Professor Rafael Br├╝schweiler of The Ohio State University, the FSU researchers used a method known as nuclear magnetic resonance, or NMR, to probe the functional dynamics of the glucokinase in great detail. This research built upon another paper Miller and Br├╝schweiler wrote in Angwandte Chemie last month that quantified the structural dynamics of both normal and disease-variant forms of glucokinase.