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Gene insertion by genome editing

Using a novel gene knock-in technique, effective insertion of an exogenous gene was demonstrated in and in , including silkworms and frogs. This strategy universally enables gene knock-in not only in , but also in various organisms.

[Knock-in Frog Embryo]
This image shows fluorescence visualization of a gill in a frog embryo using the PITCh knock-in system. The arrow indicates the gill (green).
Credit: , , Hiroshima University


Hiroshima University