Using a novel gene knock-in technique, effective insertion of an exogenous gene was demonstrated in human cells and in animal models, including silkworms and frogs. This strategy universally enables gene knock-in not only in cultured cells, but also in various organisms.
This image shows fluorescence visualization of a gill in a frog embryo using the PITCh knock-in system. The arrow indicates the gill (green).
Credit: Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University