Researchers at the University of Southampton, in collaboration with colleagues at the University of Cambridge, have developed a technique to help treat fatal diseases more effectively. Dr Sumeet Mahajan and his group at the Institute for Life Sciences at Southampton are using gold nanoprobes to identify different types of cells, so that they can use the right ones in stem cell therapies.
Stem cell therapy is in its infancy, but has the potential to change the way we treat cancer and other life-threatening diseases, by replacing damaged or diseased cells with healthy ones. One of the key limitations of stem cell therapy is identifying the right cells to use for different therapies. This fundamental problem with the treatment is being tackled by this new research.
Dr Mahajan, Senior Chemistry Lecturer in Life Science Interface, says: “Stem cells could hold the key to tackling many diseases. They develop into all the various kinds of cells needed in the body – blood, nerves and organs – but it is almost impossible to tell them apart during their initial development without complex techniques, even with the most advanced microscopes. Up to now, scientists have used intrusive fluorescent markers to tag molecules and track each cell, a process which can render them useless for therapeutic purposes anyway. By using a technique discovered at Southampton in the 1970s, known as Surface Enhanced Raman Spectroscopy (SERS), we have been able to look at adult stem cells on a molecular scale to distinguish one from another, meaning we can still use the cells for therapeutic purposes.”
The team who discovered SERS in the 1970s found that by roughening a metal surface upon which they had placed molecules to be examined, they could increase the signal by which they could detect these molecules, by a million times. This allowed them to detect molecules in far smaller quantities than ever before. SERS has been used in many different capacities around the world and across industries, but this new research marks the first time it has been used in the field of cell therapeutics. Dr Mahajan’s research could mean that stem cell and other cell-based therapies could be advanced much further than the current most common uses, such as bone marrow transplants.
Dr Mahajan comments: “Scientists studying neurodegenerative diseases such as Parkinson’s disease believe replenishing a patient’s depleted dopamine-generating cells, may be an effective treatment. However, in order to avoid fatal complications, we must be sure we are using the right type of replacement cells, which the work we are doing at Southampton is enabling us to do. In addition, the technique can also allow us to see if drugs are working effectively in cells, and can also be used to diagnose diseases as well as treat them.”
The results of Dr Mahajan’s work, funded by the Engineering and Physical Sciences Research Council (EPSRC), have been published in the influential journal Nano Letters. He is collaborating with major pharmaceutical companies to further develop more effective drugs using this technique.
The Engineering and Physical Sciences Research Council (EPSRC) which is funding Dr Mahajan’s research project, is the UK’s main agency for funding research in engineering and the physical sciences. EPSRC invests around £800 million a year in research and postgraduate training, to help the nation handle the next generation of technological change.
Intracellular SERS Nanoprobes For Distinction Of Different Neuronal Cell Types, Anna Huefner, Wei-Li Kuan, Roger A. Barker, and Sumeet Mahajan Nano Letters, 2013, 13 (6), pp 2463–2470, DOI: 10.1021/nl400448n
University of Southampton