Salk Institute scientists have developed a new reagent to map the brain’s complex network of connections that is 20 times more efficient than their previous version. This tool improves upon a technique called rabies virus tracing, which was originally developed in the Callaway lab at Salk and is commonly used to map neural connections.
A Salk team builds upon their rabies virus technology to better map neurons across large swaths of the nervous system. In a mouse brain section (thalamus), neurons providing monosynaptic inputs to cortical inhibitory neurons are traced via rabies (blue). Purple counterstaining shows surrounding cellular architecture.
Credit: Salk Institute