3 days popular7 days popular1 month popular3 months popular

Insight Into Tissue Regeneration Offered By Newt Transcriptome

Scientists have identified protein families expressed during in newts, providing the groundwork for research into whether particular sets of genes are used for the purpose. The – the map of all RNA molecules – of the is published this week in BioMed Central’s open access journal Genome Biology. is a useful model in regenerative medicine, thanks to its ability to regenerate tissue, and this data gives insights into the mechanisms behind this process.

N. viridescens, the common newt, is native to North America, and an urodelian amphibian. Newt and salamander genomes are enormous; currently too big to sequence, but their potential to regenerate entire limbs, along with parts of the central nervous system, has fascinated scientists for over 200 years. Thomas Braun, at the Max Planck Institutes, Patagonis Tsonis at the University of Dayton and their colleagues sequenced a collection of healthy and regenerated tissues from newts, and converged them into one comprehensive transcriptome. Their analysis identified 826 proteins specific for urodeles, and several newly identified proteins that they believe may play important roles in regeneration process unique for urodeles. Their data also outline genes that appear only in regenerating, but not uninjured material, which will be of interest in regenerative medicine.

The transcriptome is not complete, but serves as a matrix for further analyses. The authors believe that their findings represent only the tip of the iceberg: ‘Our data provide the groundwork for mechanistic experiments to answer the question whether urodeles utilize proprietary sets of genes for tissue regeneration.’ They continue: ‘The newly established de novo transcriptome [...] will be an indispensable resource for a better understanding of regenerative events in newts and facilitate the identifications of molecules [...] that control this fascinating process.’


A de novo assembly of the combined with proteomic validation identifies new protein families expressed during tissue regeneration
Mario Looso, Jens Preussner, Konstantinos Sousounis, Marc Bruckskotten, Christian S. Michel, Ettore Lignelli, Richard Reinhardt, Sabrina Höffner, Marcus Krüger, Panagiotis A. Tsonis3, Thilo Borchardt and Thomas Braun
Genome Biology (in press)
BioMed Central