3 days popular7 days popular1 month popular3 months popular

Need for blood donations to maintain platelet supply may be eliminated by new stem cell method

Platelets, whose primary function is to prevent bleeding, are vital for treating various forms of trauma and blood diseases. However, they can only be obtained through at present. Researchers reporting online in the Cell Press journal Cell recently found a way to create platelets without the need for donated blood, an advance that could possibly erase supply shortages and ensure platelet treatments for all who need them.

The supply of donated platelets, which have a short shelf life and must be kept at room temperature, is often insufficient to meet clinical needs. In addition, while transfused platelets do not typically need to be immune-matched to patients, repeated transfusion of unmatched platelets leads to an immune reaction that eventually renders patients unresponsive to therapy.

To address these limitations, investigators from Japan developed a strategy to derive functional platelets from human induced pluripotent . Induced pluripotent can be generated from various types of cells in the body, and they can in turn be coaxed to develop into nearly any other cell type. In the current study, the approach involved genetically manipulating such to become stable immortalized lines of platelet-producing cells called megakaryocyte progenitors.

The megakaryocyte progenitors could produce large quantities of platelets with clotting capabilities that were similar to those of donated platelets. Unlike freshly donated platelets, though, the immortalized megakaryocyte progenitors could be expanded and frozen for long-term storage.

“Here we established a method to achieve the long-term self-replication of megakaryocyte progenitors as an immortalized cell line, which could eventually contribute to large-scale cultivation and production of platelets,” says senior author Dr. Koji Eto of Kyoto University and the University of Tokyo.

Source

Cell Stem Cell, Nakamura et al.: “Expandable megakaryocyte cell lines enable clinically-applicable generation of platelets from human induced .”

Cell Press