Preventing infection in hosts receiving transplanted fetal tissues for neurodegenerative disease damage repair
The use of central nervous system fetal tissues derived from routine elective abortions to provide stem cells for transplantation procedures aimed at restoring damage done by neurodegenerative diseases is an established therapy. However, fetal tissue microbial contaminants have been known to cause brain infections in cell transplantation recipients. Now, a research team from Germany has developed a “washing” technique that decontaminates the fetal tissues from which stem cells are derived.
The study appears as an early e-publication for the journal Cell Transplantation, and is now freely available on-line.*
“Fetal neural tissue transplantation has become a potential treatment option for patients suffering from neurodegenerative diseases such as Parkinson’s disease (PD) and Huntington’s disease (HD),” explained study co-author Dr. Guido Nikkah of the Department of Neurosurgery, Erlangen University Clinic, Erlangen, Germany. “However, in Europe neural transplantation of fetal tissue must be performed in accordance with guidelines on “Good Manufacturing Processes,” which means that aspects of microbiological safety have to be investigated in detail.”
According to the researchers, aborted fetal human tissues carry the risk of bacterial contamination from the abortion process and this is the contributing factor to the subsequent risk of infection to cell recipients. The researchers noted that prior to their study it was not clear whether fetal tissue contamination, or subsequent surgical contamination during cell transplantation normally had the greatest impact as potential contributing factors to microbial infection in hosts.
Their study revealed a wide range of microbial contamination in fetal tissues that could cause infections. 47.7 % of the fetal tissues they sampled were positive for microbial infection. They also found that the risk of bacterial infection was higher than the risk of fungal infection.
To prevent microbial infection in cell transplantation hosts, the researchers developed and tested a microbial “washing” technique. In laboratory tests using rats as transplantation hosts, the technique has been shown to eliminate microbial contamination. Based on the technique’s success in decontaminating fetal tissue cells transplanted into laboratory animals, the washing technique was subsequently used in clinical practice.
“To date, more than 350 PD and approximately 100 HD patients have received primary fetal grafts, and so far no contamination has been reported,” said Dr. Nikkah.
The researchers noted that although the risk of infection is very low after their procedure, they still recommend antibiotic prophylaxis.
“Detection of a relevant microbial contamination within the narrow time-window prior to transplantation remains difficult,” concluded the authors. “While contamination of the transportation fluid is not an exclusion criterion for tissue transplantation, detection of contamination after washing steps is critical.”
“The potentially deleterious impact of microbial contamination on the use of cells or tissues for transplantation is an important health factor that cannot be ignored.” said Dr. Shinn-Zong Lin, professor of Neurosurgery and superintendent at the China Medical University Hospital, Beigang, Taiwan. “Procedures to reduce the likelihood of contamin
ation such as that described within this paper are therefore of great importance.”
Citation: Piroth, T.; Pauly, M. C.; Schneider, C.; Wittmer, A.; Mollers, S.; Dobrossy, M.; Winkler, C.; Nikkhah, G. Transplantation of human fetal tissue for neurodegenerative diseases : Validation of a new protocol for microbiological analysis and bacterial decontamination. Cell Transplantation. Appeared or available online: April 29, 2013
The Coeditors-in-chief for CELL TRANSPLANTATION are at the Diabetes Research Institute, University of Miami Miller School of Medicine and Center for Neuropsychiatry, China Medical University Hospital, Beigang, Taiwan.
Cell Transplantation Center of Excellence for Aging and Brain Repair