Viruses that infect bacteria are among the most abundant life forms on Earth. Our oceans and soils, and potentially even our own bodies, would be overrun with bacteria were it not for bacteria-eating viruses – called bacteriophages – that keep the microbial balance in check.
Now, a new study at Washington University School of Medicine in St. Louis suggests that bacteriophages made of RNA – a close chemical cousin of DNA – likely play a much larger role in shaping the bacterial makeup of worldwide habitats than previously recognized.
The research, publishing in the Open Access journal PLOS Biology, identifies 122 new types of RNA bacteriophages in diverse ecological niches, providing an opportunity to define their contributions to ecology, and potentially to fight bacterial infections, particularly those resistant to antibiotics.
“Lots of DNA bacteriophages have been identified, but there’s an incredible lack of understanding about RNA bacteriophages,” explained senior author David Wang, PhD, associate professor of molecular microbiology. “They have been largely ignored – relatively few were known to exist, and for the most part scientists haven’t bothered to look for them. This study puts RNA bacteriophages on the map and opens many new avenues of exploration.”
Wang estimates that of the more than 1,500 bacteriophages that have been identified, 99 percent of them have DNA genomes. The advent of large-scale genome sequencing has helped scientists identify DNA bacteriophages in the human gut, skin and blood as well as in the environment, but few researchers have looked for RNA bacteriophages in those samples.
First author Siddharth Krishnamurthy and the team, including Dan Barouch, MD, PhD, Beth Israel Deaconess Medical Center and Harvard Medical School, identified RNA bacteriophages by analyzing data from oceans, sewage, soils, crabs, sponges and barnacles, as well as insects, mice and rhesus macaques.
RNA bacteriophages have been shown to infect gram-negative bacteria, which have become increasingly resistant to antibiotics and are the source of many infections in health care settings. But the researchers also showed for the first time that these bacteriophages may also infect gram-positive bacteria, responsible for strep and staph infections as well as MRSA.
“What we know about RNA bacteriophages in any environment is limited,” Wang said. “But you can think of bacteriophages and bacteria as having a predator-prey relationship. We need to understand the dynamics of that relationship. Eventually, we’d like to manipulate that dynamic to use phages to selectively kill particular bacteria.”