The surface characteristics of nanoscaffolds made by nanotechnology are more similar to the three-dimensional topological structure of the extracellular matrix and the effects on the biological behaviors of cells and tissue repair are more beneficial.
Dr. Jihui Zhou and team from the Fifth Hospital Affiliated to Qiqihar Medical University prepared aligned and randomly oriented collagen nanofiber scaffolds using electronic spinning technology. The diameters and appearance of prepared scaffolds reached the standards of tissue-engineered nanometer scaffolds. The nanofiber scaffolds were characterized by a high swelling ratio, high porosity and good mechanical properties. The proliferation of spinal cord-derived neural stem cells on novel nanofiber scaffolds was obviously enhanced. The expression levels of cyclin D1 and cyclin-dependent kinase 2 were increased. Bcl-2 expression was significantly increased, but Bax and caspase-3 gene expressions were obviously decreased. There was no significant difference in the differentiation of neural stem cells into neurons on aligned and randomly oriented collagen nanofiber scaffolds.
These results, which were published in Neural Regeneration Research (Vol. 8, No. 16, 2013), indicate that novel nanofiber scaffolds could promote the proliferation of spinal cord-derived neural stem cells and inhibit apoptosis without inducing differentiation. Nanofiber scaffolds regulate apoptosis and proliferation in neural stem cells by altering gene expression.
Article: ” Novel nanometer scaffolds regulate the biological behaviors of neural stem cells,” by Jihui Zhou1, Fuge Sui1, Meng Yao2, Yansong Wang2, Yugang Liu2, Feipeng Tian1, Qiang Li1, Xiaofeng He1, Lin Shao1, Zhiqiang Liu1 (1 Longnan Hospital of Daqing, i.e. the Fifth Hospital Affiliated to Qiqihar Medical University, Daqing 163453, Heilongjiang Province, China; 2 Department of Spine Surgery, the Second Hospital Affiliated to Harbin Medical University, Harbin 150086, Heilongjiang Province, China)
Zhou JH, Sui FG, Yao M, Wang YS, Liu YG, Tian FP, Li Q, He XF, Shao L, Liu ZQ. Novel nanometer scaffolds regulate the biological behaviors of neural stem cells. Neural Regen Res. 2013;8(16):1455-1464.