Bioengineers at the University of California, San Diego have discovered that mouse embryos are contemplating their cellular fates in the earliest stages after fertilization when the embryo has only two to four cells, a discovery that could upend the scientific consensus about when embryonic cells begin differentiating into cell types. Their research, which used single-cell RNA sequencing to look at every gene in the mouse genome, was published recently in the journal Genome Research. In addition, this group published a paper on analysis of “time-course”single-cell data which is taken at precise stages of embryonic development in the journal of Proceedings of the National Academy of Sciences.
The research team used single-cell RNA-sequencing to measure every gene in the mouse genome at multiple stages of development to find differences in gene expression at precise stages.
Credit: Art by Victor O. Leshyk provided courtesy of bioeningeering professor Sheng Zhong, UC San Diego Jacobs School of Engineering.