Using a fluorescent microscope and 3D diffusion modeling researchers can see how unfolded proteins move in the cell
When a large protein unfolds in transit through a cell, it slows down and can get stuck in traffic. Using a specialized microscope – a sort of cellular traffic camera – University of Illinois chemists now can watch the way the unfolded protein diffuses.
By looking at the dynamics of how the unfolded protein moved in the cell (A), the researchers mapped areas in the cell with different rates of diffusion (B and C).
Credit: Photo courtesy Hannah Gelman
The National Science Foundation supported this work.
The paper, “Coupled Protein Diffusion and Folding in the Cell ,”Published: December 01, 2014 DOI: 10.1371/journal.pone.0113040