Right here, we current a modified in vitro end-joining (EJ) assay to quantify EJ capability, accuracy in addition to pathway change to different end-joining (Alt-EJ) or single strand annealing (SSA). A novel transformation assay was established to particularly measure round restore merchandise, which correlate with classical EJ effectivity. The EJ assay was validated utilizing EJ-deficient mammalian cell strains (Ku80, […]
