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Measuring the Infectious Titer of Recombinant Adenovirus Using Tissue Culture Infection Dose 50% (TCID50) End-Point Dilution and Quantitative Polymerase Chain Reaction (qPCR).

Measuring the Infectious Titer of Recombinant Adenovirus Using Tissue Culture Infection Dose 50% (TCID50) End-Point Dilution and Quantitative Polymerase Chain Reaction (qPCR).

Historically, adenovirus and recombinant adenovirus infectious titers have been measured by plaque assay, during which the cells are contaminated with serially diluted adenovirus inventory after which overlaid with agar; a plaque will type as the results of a single infectious occasion. Though this technique offers a quantitative readout (variety of plaques corrected for the dilution), […]

CMBs carrying PTX and CRISPR/Cas9 targeting C‑erbB‑2 plasmids interfere with endometrial cancer cells

CMBs carrying PTX and CRISPR/Cas9 targeting C‑erbB‑2 plasmids interfere with endometrial cancer cells

Improvement of mixture remedy to lower uncomfortable side effects of chemotherapeutic medication and improve their utilization fee together with gene enhancing is a key analysis subject in tumor remedy. The current research aimed to analyze the impact of cationic microbubbles (CMBs) carrying paclitaxel (PTX) and C‑erbB‑2 knockout plasmid on the endometrial most cancers cell line HEC‑1A and […]