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Co-targeting strategy for precise, scarless gene editing with CRISPR/Cas9 and donor ssODNs in Chlamydomonas

Co-targeting strategy for precise, scarless gene editing with CRISPR/Cas9 and donor ssODNs in Chlamydomonas

Programmable site-specific nucleases, such because the clustered often interspaced brief palindromic repeat (CRISPR)/ CRISPR-associated protein 9 (Cas9) ribonucleoproteins (RNPs), have allowed creation of priceless knockout mutations and focused gene modifications in Chlamydomonas (Chlamydomonas reinhardtii). Nonetheless, in walled strains, current strategies for modifying genes missing a selectable phenotype contain co-transfection of RNPs and exogenous double-stranded DNA (dsDNA) encoding […]