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Efficient manipulation of gene dosage in human iPSCs using CRISPR/Cas9 nickases

Efficient manipulation of gene dosage in human iPSCs using CRISPR/Cas9 nickases

The dysregulation of gene dosage as a consequence of duplication or haploinsufficiency is a significant reason for autosomal dominant illnesses equivalent to Alzheimer’s illness. Nevertheless, there’s at the moment no fast and environment friendly methodology for manipulating gene dosage in a human mannequin system equivalent to human induced pluripotent stem cells (iPSCs). Right here, we […]

Cortisol promotes stress tolerance via DAF-16 in Caenorhabditis elegans

Cortisol promotes stress tolerance via DAF-16 in Caenorhabditis elegans

On this examine, we studied the consequences of cortisol and cortisone on the age-related lower in locomotion within the nematode Caenorhabditis elegans and on the tolerance to warmth stress at 35 °C and to oxidative stress induced by the publicity to 0.1% H2O2. Adjustments in mRNA expression ranges of C. elegans genes associated to emphasize tolerance have been additionally […]