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Administration of cyclophosphamide to rats induces pica and potentiates 5-hydroxytryptamine synthesis in the intestine without causing severe intestinal injury
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Administration of cyclophosphamide to rats induces pica and potentiates 5-hydroxytryptamine synthesis in the intestine without causing severe intestinal injury

- November 15, 2021 | November 3, 2022 - Mitchell
The consequences of cyclophosphamide on 5-hydroxytryptamine (5-HT) synthesis within the intestinal tissue of rats had been investigated. Rats acquired 120 mg/kg cyclophosphamide intraperitoneally as a single administration, and kaolin and meals consumption was measured by an computerized monitoring equipment. Ileal tissues had been collected at both 24 or 72 h after administration.
Cyclophosphamide brought about a major improve in kaolin consumption on the acute and the delayed phases and was related to a lower in meals consumption, and physique weight. Cyclophosphamide had no important impact on intestinal mucosal morphology, or inducible nitric oxide synthase and cyclooxygenase-2 expression within the gut.
Cyclophosphamide considerably elevated tryptophan hydroxylase 1 (TPH1) mRNA expression, variety of anti-TPH antibody-positive cells, and 5-HT content material within the gut. Cyclophosphamide additionally considerably elevated the expression of Tac1 mRNA, encoding preprotachykinin-1, which is a preprotein of substance P, and the variety of anti-substance P antibody-positive cells within the gut.
Cyclophosphamide considerably elevated Lgr5, Bmi1, and Atoh1 mRNA ranges, that are markers for the proliferation and differentiation of stem cells. This examine demonstrated that cyclophosphamide induced pica in rats, and potentiated 5-HT synthesis related to hyperplasia of substance P-containing enterochromaffin cells with out inflicting extreme intestinal damage.
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Characterization of iPS87, a prostate most cancers stem cell-like cell line.

Prostate most cancers impacts a whole bunch of hundreds of males and households all through the world. Though chemotherapy, radiation, surgical procedure, and androgen deprivation remedy are utilized, these therapies don’t remedy metastatic prostate most cancers. Sufferers handled by androgen deprivation usually develop castration resistant prostate most cancers which is incurable. Novel approaches of remedy are clearly needed.
We have now beforehand proven that prostate most cancers originates as a stem cell illness. A prostate most cancers affected person pattern, #87, obtained from prostatectomy surgical procedure, was collected and frozen as single cell suspension. Most cancers stem cell cultures had been grown, single cell-cloned, and proven to be tumorigenic in SCID mice.
Nonetheless, outdoors its pure area of interest, the classy prostate most cancers stem cells misplaced their tumor-inducing functionality and stem cell marker expression after roughly eight transfers at a 1:Three cut up ratio. Tumor-inducing exercise might be restored by inducing the cells to pluripotency utilizing the strategy of Yamanaka.
Cultures of human prostate-derived regular epithelial cells acquired from business sources had been equally induced to pluripotency and these didn’t purchase a tumor phenotype in vivo. To characterize the iPS87 cell line, cells had been stained with antibodies to varied markers of stem cells together with: ALDH7A1, LGR5, Oct4, Nanog, Sox2, Androgen Receptor, and Retinoid X Receptor.
These markers had been discovered to be expressed by iPS87 cells, and the excessive tumorigenicity in SCID mice of iPS87 was confirmed by histopathology. This analysis thus characterizes the iPS87 cell line as a cancer-inducing, stem cell-like cell line, which can be utilized within the improvement of novel therapies for prostate most cancers.

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