The consequences of cyclophosphamide on 5-hydroxytryptamine (5-HT) synthesis within the intestinal tissue of rats had been investigated. Rats acquired 120 mg/kg cyclophosphamide intraperitoneally as a single administration, and kaolin and meals consumption was measured by an computerized monitoring equipment. Ileal tissues had been collected at both 24 or 72 h after administration.
Cyclophosphamide brought about a major improve in kaolin consumption on the acute and the delayed phases and was related to a lower in meals consumption, and physique weight. Cyclophosphamide had no important impact on intestinal mucosal morphology, or inducible nitric oxide synthase and cyclooxygenase-2 expression within the gut.
Cyclophosphamide considerably elevated tryptophan hydroxylase 1 (TPH1) mRNA expression, variety of anti-TPH antibody-positive cells, and 5-HT content material within the gut. Cyclophosphamide additionally considerably elevated the expression of Tac1 mRNA, encoding preprotachykinin-1, which is a preprotein of substance P, and the variety of anti-substance P antibody-positive cells within the gut.
Cyclophosphamide considerably elevated Lgr5, Bmi1, and Atoh1 mRNA ranges, that are markers for the proliferation and differentiation of stem cells. This examine demonstrated that cyclophosphamide induced pica in rats, and potentiated 5-HT synthesis related to hyperplasia of substance P-containing enterochromaffin cells with out inflicting extreme intestinal damage.
Custom Antibody titration by ELISA up to 2 rabbits and 1 bleed | |
ELISA-1 | Alpha Diagnostics |
Beta2-Microglobulin ELISA kit ELISA Kit | |
LF-EK60047 | Abfrontier |
Chicken thrombomodulin,TM ELISA KIT ELISA | |
QY-E80092 | Qayee Biotechnology |
Oxycodone ELISA | |
EK7130 | BosterBio |
Amphiphysin ELISA | |
LF-EK0189 | Abfrontier |
Characterization of iPS87, a prostate most cancers stem cell-like cell line.
Prostate most cancers impacts a whole bunch of hundreds of males and households all through the world. Though chemotherapy, radiation, surgical procedure, and androgen deprivation remedy are utilized, these therapies don’t remedy metastatic prostate most cancers. Sufferers handled by androgen deprivation usually develop castration resistant prostate most cancers which is incurable. Novel approaches of remedy are clearly needed.
We have now beforehand proven that prostate most cancers originates as a stem cell illness. A prostate most cancers affected person pattern, #87, obtained from prostatectomy surgical procedure, was collected and frozen as single cell suspension. Most cancers stem cell cultures had been grown, single cell-cloned, and proven to be tumorigenic in SCID mice.
Nonetheless, outdoors its pure area of interest, the classy prostate most cancers stem cells misplaced their tumor-inducing functionality and stem cell marker expression after roughly eight transfers at a 1:Three cut up ratio. Tumor-inducing exercise might be restored by inducing the cells to pluripotency utilizing the strategy of Yamanaka.
Cultures of human prostate-derived regular epithelial cells acquired from business sources had been equally induced to pluripotency and these didn’t purchase a tumor phenotype in vivo. To characterize the iPS87 cell line, cells had been stained with antibodies to varied markers of stem cells together with: ALDH7A1, LGR5, Oct4, Nanog, Sox2, Androgen Receptor, and Retinoid X Receptor.
These markers had been discovered to be expressed by iPS87 cells, and the excessive tumorigenicity in SCID mice of iPS87 was confirmed by histopathology. This analysis thus characterizes the iPS87 cell line as a cancer-inducing, stem cell-like cell line, which can be utilized within the improvement of novel therapies for prostate most cancers.

Custom Antibody titration by ELISA up to 2 rabbits and 1 bleed | |||
Alpha Diagnostics | |||
Beta2-Microglobulin ELISA kit ELISA Kit | |||
Abfrontier | |||
Chicken thrombomodulin,TM ELISA KIT ELISA | |||
Qayee Biotechnology |
p-Ezrin (Thr567) regulates Hippo pathway and Yap in liver.
Earlier research have proven that activation of CD81, (the portal of entry of Hepatitis C virus) by agonistic antibody leads to phosphorylation of Ezrin through Syk kinase. We have now beforehand that his is related to inactivation of the Hippo pathway and improve in YAP. The alternative happens when Glypican-3 (GPC3) or E2 protein of HCV bind to CD81.
Hepatocyte-specific GPC3 transgenic mice have decreased p-Ezrin(Thr567) and Yap, elevated Hippo exercise and suppressed liver regeneration. The position of Ezrin in these processes has been speculated, however not confirmed. We now present proof for a direct position of Ezrin in regulation of Hippo pathway and Yap.
Pressured expression of plasmids expressing mutant Ezrin (T567D) (mimicking p-Ezrin(Thr567)) suppressed Hippo exercise and activated Yap signaling in hepatocytes in vivo, and enhanced activation of pathways of beta catenin and LGR4 and LGR5 receptors. Hepatoma cell traces JM1 and JM2 have decreased CD81 expression and Hippo exercise, and up regulated pEzrin(T567). NSC668394, a p-Ezrin(Thr567) antagonist, brought about important lower in hepatoma cell proliferation. We moreover current proof that pEzrin(T567) is managed by EGFR and MET.
Ezrin phosphorylation, mediated by CD81-associated Syk kinase, is immediately concerned in regulation of Hippo pathway, Yap ranges and development of regular and neoplastic hepatocytes. The discovering has mechanistic and probably therapeutic functions in hepatocytes development biology and HCC and HCV pathogenesis.
Custom Antibody titration by ELISA up to 2 rabbits and 1 bleed | |||
ELISA-1 | |||
Beta2-Microglobulin ELISA kit ELISA Kit | |||
LF-EK60047 | |||
Chicken thrombomodulin,TM ELISA KIT ELISA | |||
QY-E80092 |
Canine Epithelial Pores and skin Tumours: Expression of the Stem Cell Markers Lgr5, Lgr6 and Sox9 in Gentle of New Most cancers Stem Cell Theories.
Proof is accumulating that tumour improvement is pushed by most cancers stem cells (CSCs). So as to perceive the presence and potential contribution of stem cells (SCs) as tumour-initiating cells in canine cutaneous tumours, we chosen three putative SC markers (Lgr5, Lgr6 and Sox9) and investigated their expression sample, degree of protein and mRNA expression, in 43 canine hair follicle (HF) and 18 canine cutaneous epidermal tumours by immunohistochemistry and qRT-PCR, utilizing regular pores and skin samples as controls. Lgr5 protein expression was not detected in epidermal and HF tumours; nevertheless, Lgr5 mRNA overexpression was evident in some HF tumours.
Sox9 was expressed in a number of tumour circumstances, each on the protein and mRNA degree. The Lgr6 antibody examined was not appropriate for formalin-fixed paraffin-embedded tissue samples, however Lgr6 gene confirmed larger expression in a number of samples of each HF and epidermal tumours in contrast with regular pores and skin.
Considerably larger mRNA expression ranges of the three SC markers had been present in trichoblastomas (TB) in contrast with basal cell carcinomas (BCC). The current outcomes indicated that canine HF and epidermal tumours might need frequent tumour-initiating cells. The mRNA expression of the three chosen SC markers, particularly Lgr5, might be probably helpful within the distinction between canine TB and BCC.
Identification and Isolation of Human LGR5+ Cells Utilizing an Antibody-Primarily based Technique
Leucine-rich repeat-containing G protein-coupled receptor 5 (Lgr5) has been recognized as a marker of stem cells throughout a number of tissues. Lgr5-expressing cells are additionally regulators of tissue homeostasis and wound restore, and drivers of carcinogenic development. Nearly all of details about Lgr5-expressing cells derives from genetically engineered mouse fashions.
PCR Tubes (50) | |||
PCR-50 | |||
MULTIPLEX KIT PCR MASTITIS PCR kit | |||
PCR-MPX218-48D | |||
MULTIPLEX KIT PCR MASTITIS PCR kit | |||
PCR-MPX218-96D |
Human research have been restricted by an absence of particular reagents and experimental procedures for the purification of those cells. We lately demonstrated that antibody-based purification can be utilized to acquire viable LGR5-expressing cells from human main tissues and affected person derived organoids.
LGR5 Antibody |
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CSB-PA622827-100ul | Cusabio | 100ul | EUR 379.2 |
Description: A polyclonal antibody against LGR5. Recognizes LGR5 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000 |
LGR5 Antibody |
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DF2816 | Affbiotech | 200ul | EUR 420 |
LGR5 Antibody |
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AF4102 | Affbiotech | 200ul | EUR 540 |
LGR5 Antibody |
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1-CSB-PA012906LA01HU | Cusabio |
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Description: A polyclonal antibody against LGR5. Recognizes LGR5 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC, IF; Recommended dilution: IHC:1:200-1:500, IF:1:50-1:200 |
LGR5 Antibody |
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F48167-0.4ML | NSJ Bioreagents | 0.4 ml | EUR 379 |
LGR5 Antibody |
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A1007-100 | Biovision | each | EUR 424.8 |
LGR5 Antibody |
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CSB-PA622827- | Cusabio | each | EUR 402 |
Description: A polyclonal antibody against LGR5. Recognizes LGR5 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000 |
LGR5/GPR49 Antibody |
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48810-100ul | SAB | 100ul | EUR 399.6 |
LGR5/GPR49 Antibody |
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48810-50ul | SAB | 50ul | EUR 286.8 |
LGR5(GPR49) Antibody |
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21659-100ul | SAB | 100ul | EUR 302.4 |
LGR5(GPR49) Antibody |
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21659-50ul | SAB | 50ul | EUR 224.4 |
LGR5 Polyclonal Antibody |
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ABP59113-003ml | Abbkine | 0.03ml | EUR 189.6 |
Description: A polyclonal antibody for detection of LGR5 from Human, Mouse. This LGR5 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human LGR5 protein |
LGR5 Polyclonal Antibody |
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ABP59113-01ml | Abbkine | 0.1ml | EUR 346.8 |
Description: A polyclonal antibody for detection of LGR5 from Human, Mouse. This LGR5 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human LGR5 protein |
LGR5 Polyclonal Antibody |
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ABP59113-02ml | Abbkine | 0.2ml | EUR 496.8 |
Description: A polyclonal antibody for detection of LGR5 from Human, Mouse. This LGR5 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human LGR5 protein |
LGR5 (GPR49) Antibody |
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abx020041-100ug | Abbexa | 100 ug | EUR 526.8 |
LGR5 / GPR49 Antibody |
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abx031681-400ul | Abbexa | 400 ul | EUR 627.6 |
LGR5 / GPR49 Antibody |
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abx031681-80l | Abbexa | 80 µl | EUR 343.2 |
LGR5 (loop2) Antibody |
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abx031683-400ul | Abbexa | 400 ul | EUR 627.6 |
LGR5 (loop2) Antibody |
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abx031683-80l | Abbexa | 80 µl | EUR 343.2 |
LGR5 (GPR49) Antibody |
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ABF4102 | Lifescience Market | 100 ug | EUR 525.6 |
Polyclonal LGR5 Antibody |
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APR00432G | Leading Biology | 0.1ml | EUR 580.8 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human LGR5 . This antibody is tested and proven to work in the following applications: |
LGR5 Conjugated Antibody |
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C44974 | SAB | 100ul | EUR 476.4 |
LGR5 Polyclonal Antibody |
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ES11504-100ul | ELK Biotech | 100ul | EUR 334.8 |
Description: A Rabbit Polyclonal antibody against LGR5 from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA |
LGR5 Polyclonal Antibody |
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ES11504-50ul | ELK Biotech | 50ul | EUR 248.4 |
Description: A Rabbit Polyclonal antibody against LGR5 from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA |
Anti-LGR5 antibody |
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PAab04765 | Lifescience Market | 100 ug | EUR 494.4 |
anti- LGR5 antibody |
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FNab04765 | FN Test | 100µg | EUR 702 |
Description: Antibody raised against LGR5 |
Anti-LGR5 antibody |
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STJ11100682 | St John's Laboratory | 50 µl | EUR 344.4 |
Description: The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene. |
Anti-LGR5 antibody |
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STJ112563 | St John's Laboratory | 100 µl | EUR 332.4 |
Description: The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene. |
Anti-LGR5 antibody |
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STJ192662 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Unconjugated Rabbit polyclonal to LGR5 |
LGR5 Antibody / GPR49 |
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RQ4782 | NSJ Bioreagents | 100ul | EUR 419 |
LGR5 Antibody (GPR49) |
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F40329-0.08ML | NSJ Bioreagents | 0.08 ml | EUR 165 |
LGR5 Antibody (GPR49) |
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F48167-0.08ML | NSJ Bioreagents | 0.08 ml | EUR 165 |
LGR5 Antibody (GPR49) |
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F48168-0.05ML | NSJ Bioreagents | 0.05 ml | EUR 165 |
Right here, we offer detailed strategies for the purification of those cells from colonic epithelial organoids generated from patient-derived tissues, from induced pluripotent stem cell (iPSC) derived intestinal organoids, and from freshly remoted affected person tissue intestinal crypts. These strategies will facilitate experimental evaluation of human LGR5-expressing cells in improvement, wound therapeutic, and most cancers.
Custom Antibody titration by ELISA up to 2 rabbits and 1 bleed | |||
ELISA-1 | |||
Beta2-Microglobulin ELISA kit ELISA Kit | |||
LF-EK60047 | |||
Chicken thrombomodulin,TM ELISA KIT ELISA | |||
QY-E80092 |
Custom Antibody titration by ELISA up to 2 rabbits and 1 bleed | |||
Alpha Diagnostics | |||
Beta2-Microglobulin ELISA kit ELISA Kit | |||
Abfrontier | |||
Chicken thrombomodulin,TM ELISA KIT ELISA | |||
Qayee Biotechnology | |||
Oxycodone ELISA | |||
BosterBio | |||
Amphiphysin ELISA | |||
Abfrontier |
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