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Modulation of Titin-Based Stiffness in Hypertrophic Cardiomyopathy via Protein Kinase D.

Modulation of Titin-Based Stiffness in Hypertrophic Cardiomyopathy via Protein Kinase D.

The large protein titin performs structure-preserving capabilities within the sarcomere and is vital for the passive stiffness (Fpassive) of cardiomyocytes. Protein kinase D (PKD) enzymes play essential roles in regulating myocardial contraction, hypertrophy, and reworking.
PKD phosphorylates myofilament proteins, however it’s not recognized whether or not the enormous protein titin can also be a PKD substrate. Right here, we aimed to find out whether or not PKD phosphorylates titin and thereby modulates cardiomyocyte Fpassive in regular and failing myocardium. The phosphorylation of titin was assessed in cardiomyocyte-specific PKD knock-out mice (cKO) and human hearts utilizing immunoblotting with a phosphoserine/threonine and a phosphosite-specific titin antibody.
PKD-dependent site-specific titin phosphorylation in vivo was quantified by mass spectrometry utilizing steady isotope labeling by amino acids in cell tradition (SILAC) of SILAC-labeled mouse coronary heart protein lysates that have been blended with lysates remoted from hearts of both wild-type management (WT) or cKO mice. Fpassive of single permeabilized cardiomyocytes was recorded earlier than and after PKD and HSP27 administration.
All-titin phosphorylation was lowered in cKO in comparison with WT hearts. A number of conserved PKD-dependent phosphosites have been recognized inside the Z-disk, A-band and M-band areas of titin by quantitative mass spectrometry, and plenty of PKD-dependent phosphosites detected within the elastic titin I-band area have been considerably decreased in cKO.
Evaluation of titin site-specific phosphorylation confirmed unaltered or upregulated phosphorylation in cKO in comparison with matched WT hearts. Fpassive was elevated in cKO in comparison with WT cardiomyocytes and PKD administration lowered Fpassive of WT and cKO cardiomyocytes. Cardiomyocytes from hypertrophic cardiomyopathy (HCM) sufferers confirmed greater Fpassive in comparison with management hearts and considerably decrease Fpassive after PKD remedy.
As well as, we discovered greater phosphorylation at CaMKII-dependent titin websites in HCM in comparison with management hearts. Expression and phosphorylation of HSP27, a substrate of PKD, have been elevated in HCM hearts, which was related to elevated PKD expression and phosphorylation.
The relocalization of HSP27 in HCM away from the sarcomeric Z-disk and I-band urged that HSP27 didn’t exert its protecting motion on titin extensibility. This safety might, nevertheless, be restored by administration of HSP27, which considerably lowered Fpassive in HCM cardiomyocytes. These findings set up a beforehand unknown position for PKDin regulating diastolic passive properties of wholesome and diseased hearts.

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Examine the Phosphoproteome in Prostate Most cancers.

Phosphoproteomics includes the large-scale examine of phosphorylated proteins. Protein phosphorylation is a vital step in lots of sign transduction pathways and is tightly regulated by kinases and phosphatases. Subsequently, characterizing the phosphoproteome might present insights into figuring out novel targets and biomarkers for oncologic remedy.
Mass spectrometry gives a strategy to globally detect and quantify hundreds of distinctive phosphorylation occasions. Nonetheless, phosphopeptides are a lot much less considerable than non-phosphopeptides, making biochemical evaluation more difficult. To beat this limitation, strategies to complement phosphopeptides previous to the mass spectrometry evaluation are required.
We describe a process to extract and digest proteins from tissue to yield peptides, adopted by an enrichment for phosphotyrosine (pY) and phosphoserine/threonine (pST) peptides utilizing an antibody-based and/or titanium dioxide (TiO2)-based enrichment technique. After the pattern preparation and mass spectrometry, we subsequently determine and quantify phosphopeptides utilizing liquid chromatography-mass spectrometry and evaluation software program.

Protein phosphorylation throughout coconut zygotic embryo growth

Proof was obtained on the incidence of protein threonine, serine, and tyrosine (Tyr) kinases in creating coconut (Cocos nucifera L. ) zygotic embryos, primarily based on in vitro phosphorylation of proteins within the presence of gamma-32PATP, alkaline remedy, and thin-layer chromatography evaluation, which confirmed the presence of 32Pphosphoserine, 32Pphosphothreonine, and 32Pphosphotyrosine in 32P-labeled protein hydrolyzates.
Tyr kinase exercise was additional confirmed in extracts of embryos at totally different phases of growth utilizing antiphosphotyrosine monoclonal antibodies and the artificial peptide derived from the amino acid sequence surrounding the phosphorylation web site in pp60(src) (RR-SRC), which is particular for Tyr kinases.
Anti-phosphotyrosine western blotting revealed a altering profile of Tyr-phosphorylated proteins throughout embryo growth. Tyr kinase exercise, as assayed utilizing RR-SRC, additionally modified throughout embryo growth, exhibiting two peaks of exercise, one throughout early and one other throughout late embryo growth. As well as, using genistein, a Tyr kinase inhibitor, diminished the power of extracts to phosphorylate RR-SRC.
Outcomes offered right here present the incidence of threonine, serine, and Tyr kinases in creating coconut zygotic embryos, and recommend that protein phosphorylation, and the doable inference of Tyr phosphorylation specifically, might play a task within the coordination of the event of embryos on this species.
Modulation of Titin-Based Stiffness in Hypertrophic Cardiomyopathy via Protein Kinase D.

Phosphorylation of the nucleocapsid protein of Hantaan virus by casein kinase II.

Hantaanvirus (HTNV) is the prototype of the genus Hantavirus, which belongs to the household Bunyaviridae. Hantaviruses are carried and transmitted by rodents and are recognized to trigger two severe illness syndromes in people i.e., hemorrhagic fever with renal syndrome (HFRS) and the hantavirus pulmonary syndrome (HPS).
HTNV is an enveloped virus that comprises a tripartite genome consisting of three negative-sense RNA segments (L, M, S), and the S and M section of HTNV, respectively, encode the viral nucleocapsid protein (NP) and envelope glycoproteins.
Attainable phosphorylation motifs of casein kinase II (CKII) and protein kinase C (PKC) have been recognized in HTNV NP via bioinformatics searches. Sucrose gradient SDS-PAGE evaluation indicated that dephosphorylated HTNV NP migrated sooner than non-dephosphorylated NP, suggesting that HTNV NP is phosphorylated in contaminated Vero E6 cells.
Immunoblot anaylsis of HTNV particles with anti-phosphoserine antibody and anti-phosphothreonine antibody after immunoprecipitation confirmed that viral particles are readily phosphorylated at threonine residues. In vitro kinase assay additional confirmed that HTNV NP is phosphorylated by CK II, however not by PKC.
Full size or truncated HTNV NPs expressed in E. coli have been phosphorylated in vitro by CKII suggesting that phosphorylation might happen in vivo at a number of websites. Web site particular mutagenesis research recommend that HTNV NP phosphorylation would possibly happen at unknown websites excluding the site-directly mutagenized areas. Taken collectively, HTNV NP could be phosphorylated primarily at threonine residues in vivo by CK II remedy.

Cryopreservation of widespread carp (Cyprinus carpio L.) sperm induces protein phosphorylation in tyrosine and threonine residues.

The impact of cryopreservation on the protein phosphorylation/dephosphorylation sample of widespread carp (Cyprinus carpio) sperm is described. Sperm was diluted in dimethyl sulfoxide (DMSO) and ethylene glycol (EG)-based extenders, adopted by equilibration, freezing, and thawing.
Proteins extracted from contemporary and cryopreserved spermatozoa have been separated on SDS-PAGE and two-dimensional gel electrophoresis, blotted on polyvinylidene difluoride membrane, and handled with anti-phosphotyrosine, anti-phosphothreonine, or anti-phosphoserine antibodies. For the following protein identification we used matrix-associated laser desorption/ionization time-of-flight mass spectrometry.
The outcomes demonstrated that cryopreservation with both DMSO or EG extender considerably altered the phosphorylation state of sperm proteins on tyrosine or threonine residues. A dramatic lower in tyrosine phosphorylation was detected within the cryopreservation procedures with DMSO extender.
Endoplasmin, transketolase, and S-adenosylhomocysteine hydrolase have been recognized as proteins that play a key position in mobile stress responses and oxidation and/or discount reactions.

Phosphoserine Antibody

abx020673-100ug 100 ug
EUR 1066
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Phosphoserine Antibody

20-abx134715
  • EUR 356.00
  • EUR 537.00
  • EUR 217.00
  • 100 ul
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Phosphoserine Antibody

20-abx159643
  • EUR 272.00
  • EUR 230.00
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Phosphoserine Antibody

abx448438-400ul 400 ul
EUR 537
  • Shipped within 5-12 working days.

Phosphoserine antibody (biotin)

60R-PR001bt 100 ug
EUR 840
Description: Rabbit polyclonal Phosphoserine antibody (biotin) conjugated

Phosphoserine Antibody (APC)

abx448359-400ul 400 ul
EUR 592
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Phosphoserine Antibody (Biotin)

abx448367-400ul 400 ul
EUR 592
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Phosphoserine Antibody (FITC)

abx448373-400ul 400 ul
EUR 578
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Phosphoserine Antibody (PerCP)

abx448393-400ul 400 ul
EUR 592
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Phosphoserine Antibody (RPE)

abx448401-400ul 400 ul
EUR 592
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Phosphoserine Antibody (Streptavidin)

abx448409-400ul 400 ul
EUR 592
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Phosphoserine Antibody (HRP)

abx448439-400ul 400 ul
EUR 606
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Phosphoserine (pSer) Antibody

abx415747-50ug 50 ug
EUR 787
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Phosphoserine Monoclonal Antibody

ABM40198-003ml 0.03ml
EUR 158
  • Immunogen information: Purified Protein
  • Applications tips:
Description: A monoclonal antibody for detection of Phosphoserine from All. This Phosphoserine antibody is for WB, IHC-P. It is affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen Purified Protein

Phosphoserine Monoclonal Antibody

ABM40198-01ml 0.1ml
EUR 289
  • Immunogen information: Purified Protein
  • Applications tips:
Description: A monoclonal antibody for detection of Phosphoserine from All. This Phosphoserine antibody is for WB, IHC-P. It is affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen Purified Protein

Phosphoserine Monoclonal Antibody

ABM40198-02ml 0.2ml
EUR 414
  • Immunogen information: Purified Protein
  • Applications tips:
Description: A monoclonal antibody for detection of Phosphoserine from All. This Phosphoserine antibody is for WB, IHC-P. It is affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen Purified Protein

Phosphoserine Monoclonal Antibody

EM1189-100ul 100ul
EUR 279
Description: A Mouse Monoclonal antibody against Phosphoserine from All. This antibody is tested and validated for WB, ELISA

Phosphoserine Monoclonal Antibody

EM1189-50ul 50ul
EUR 207
Description: A Mouse Monoclonal antibody against Phosphoserine from All. This antibody is tested and validated for WB, ELISA

Antibody for Phosphoserine

SPC-149F 0.4ml
EUR 362
  • Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases,
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Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is unconjugated.

Antibody for Phosphoserine

SPC-149F-A390 0.4ml
EUR 409
  • Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases,
  • Show more
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to ATTO 390.

Antibody for Phosphoserine

SPC-149F-A488 0.4ml
EUR 408
  • Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases,
  • Show more
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to ATTO 488.

Antibody for Phosphoserine

SPC-149F-A565 0.4ml
EUR 408
  • Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases,
  • Show more
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to ATTO 565.
Outcomes point out that the phosphorylation and/or dephosphorylation modifications of sperm proteins that happen throughout cryopreservation might stimulate a sequence of biochemical results interfering with spermatozoa operate and resulting in a lack of motility and fertilization capability. Our findings indicated that use of EG extender supplied superior protein preservation throughout sperm storage.

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