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Phospho-specific antibodies targeting the amino terminus of the human dopamine transporter.

Phospho-specific antibodies targeting the amino terminus of the human dopamine transporter.

The dopamine transporter (DAT), which mediates the inactivation of launched dopamine by means of its reuptake, is the first molecular goal for the actions of psychostimulants. An rising variety of research assist an important position for phosphorylation of serines (Ser) within the distal amino (N) terminus of DAT in regulating its perform.
Nonetheless, the molecular particulars of the regulation of phosphorylation and its affect on perform aren’t absolutely understood. To deal with this, we now have developed and characterised two distinct phospho-antibodies that acknowledge human DAT when it’s phosphorylated at Ser7 or Ser12.
Our information present that remedy of cells with phorbol 12-myristate 13-acetate (PMA), amphetamine (AMPH) or okadaic acid (OA) results in a rise within the phosphorylation of DAT at each residues and that these responses are depending on the exercise of protein kinase C.
We additionally present that AMPH-induced and OA-induced phosphorylation of DAT are depending on Ca2+/calmodulin-dependent protein kinase α. Our information additional counsel that the lipid raft localization of DAT is important for environment friendly N-terminal phosphorylation and for the related behavioral results of AMPH, demonstrating the potential of those novel antibodies as highly effective instruments to review DAT regulation and performance in vivo.

Recognition of dopamine transporters by antipeptide antibodies.

Hemocyanin-conjugated peptides comparable to amino acid sequences from 14 completely different areas of the rat dopamine transporter had been used to immunize rabbits and the ensuing antisera had been examined for dopamine transporter recognition. Eleven of the peptide sequences produced antipeptide antibodies detectable with ELISA assays.
Sera directed towards 4 of the peptides immunoprecipitated striatal transporters photoaffinity labeled with (125)IDEEP. Antisera directed towards a peptide sequence on the C-terminal area of the transporter’s putative second extracellular loop, “peptide 5,” acknowledged photolabeled dopamine transporter protein from rat, canine, and human basal ganglia.
These sera additionally acknowledged dopamine transporters from rat striatum in immunoblots; nevertheless, no response was discovered when rat cerebellum or frontal cortical tissue was used. These outcomes present additional proof that the cloned cDNA and its deduced amino acid sequence represents the protein expressed in mind and highlights the areas of the transporter which can be promising websites for the event of high-affinity antibodies.

Inhibition of cocaine binding to the human dopamine transporter by a single chain anti-idiotypic antibody: its cloning, expression, and purposeful properties.

Typical drug growth for remedy of cocaine dependancy is drastically hindered by the intense problem in designing a selective cocaine antagonist. We employed anti-idiotypic (anti-Id) antibodies to generate cocaine antagonists. The aim of this research was to analyze the feasibility of this various method.
Herein, we describe the molecular cloning, bacterial expression, and purposeful properties of an anti-Id monoclonal antibody (mAb), designated K2-3f, which possesses an inside picture of cocaine inside its variable areas. The heavy and lightweight chain variable domains of K2-3f had been cloned by reverse transcription-polymerase chain response (RT-PCR) and a single chain antibody variable fragment (scFv) was assembled for expression in Escherichia coli.
The scFv certain to the human dopamine transporter (hDAT) with average affinity (Okay(a)=5.three x 10(6) M(-1)) and glorious mimicry of the cocaine molecule utterly inhibited cocaine binding at a molar focus intently resembling in vivo situations whereas permitting roughly 90% of equimolar dopamine uptake.
Our information counsel that the usage of anti-Id antibody as a template for era of a cocaine antagonist is a promising method effectively price pursuing. If this technique is profitable, it may very well be utilized to potential ligand-receptor interactions within the remedy of different illnesses.
Phospho-specific antibodies targeting the amino terminus of the human dopamine transporter.

Dopamine uptake by mouse neuroblastoma N1E-115 cells stably expressing human dopamine transporter is differentially inhibited by anti-idiotypic ab2beta antibodies mimicking the configuration of cocaine.

We have now efficiently constructed a mouse neuronal N1E-115 cell line stably expressing a totally purposeful human dopamine transporter (hDAT). Earlier research in our laboratory have produced a number of anti-idiotypic Ab2beta antibodies that mimic the configuration of the cocaine molecule at their antigen-combining web site.
Within the current research, we noticed that some anti-idiotypic Ab2beta antibodies inhibited dopamine uptake by the hDAT-transfected line. Every antibody confirmed a distinct stage of inhibition (between 40% and 90%). These findings counsel that the interior photographs of those antibodies can be utilized as analog peptides that will compete with cocaine for its binding web site on the transporter however not impair dopamine uptake as a lot as cocaine does.

Preparation of dopamine transporter-specific antibodies utilizing molecular cloned genes.

Dopamine transporter (DAT) performs an important position in terminating the actions of dopamines launched into the synaptic cleft. DAT can also be the goal of varied psychotropic medication corresponding to cocaine and amphetamine. On this research we ready DAT-specific antibodies utilizing the 2nd extracellular loop of rat DAT as an antigen.
The 2nd extracellular loop of the rat DAT was expressed in micro organism as a fusion protein with glutathione-S-transferase, and injected into rabbits to lift antibodies. Produced antibodies clearly acknowledged the rat DAT in ELISA, immunoblotting, and immunoprecipitation.
As anticipated from the excessive sequence homology between the rat and human DAT, the antibodies raised for the rat DAT cross-reacted with the human DAT within the immunoblotting. Contemplating the specificity for DAT with wide selection of purposes corresponding to ELISA, immunoblotting, and immunoprecipitation, these antibodies can be priceless device for understanding the pharmacological actions of dopamine transporter and drug dependancy.

Expression of a dopamine transporter in PC12h cells: an immunohistochemical research with an antipeptide antibody.

Two sorts of oligopeptides, primarily based on the amino acid sequences of No. 217-232 and 374-387 of a rat dopamine transporter, had been designed and chemically synthesized. 5 clones of the monoclonal antibodies towards these peptides had been produced with the in vitro immunization technique.
Two of them have acknowledged a protein of M(r) roughly 85,000 in a synaptosomal fraction of the rat striatum. It’s possible that the protein M(r) roughly 85,000 corresponds to a dopamine transporter within the nerve terminal of the dopamine neurons within the striatum. The expression of dopamine transporter was immunohistochemically examined with these antibodies in PC12h cells. The immunoreactivity was detected on the floor membrane of the cells.

Chemical characterization of pineal neurons in perinatal rats

Ample proof signifies that in a number of mammalian species the pineal physique incorporates neurons. In grownup white albino rats neurons aren’t current within the pineal physique; nevertheless, in perinatal rats many neurons had been described.
It was demonstrated that in grownup mammalian species the pineal neurons contained some neuropeptides and neurotransmitters corresponding to leu-enkephalin, met-enkephalin, substance-P, somatostatin and γ-aminobutiric acid. Oxytocin, vasopressin mRNAs and peptides had been additionally demonstrated.
No information can be found on the chemical nature of the neurons in perinatal rats. Within the current experiment we used immunohistochemistry to make clear this concern. After paraformaldehyde fixation frozen sections had been ready and stained for immunoreactivities of a number of neuropeptides and neurotransmitters.
Dopamine β-hydroxylase (DBH), neuropeptide-Y (NPY), vesicular acetylcholine transporter (VAChT), vesicular glutamate transporter (VGluT) and calcitonin gene-related peptide (CGRP) antibodies had been capable of stain fibers. In response to earlier information these fibers could also be sympathetic (DBH, NPY), parasympathetic (VAChT) or sensory (CGRP). VGluT antibody might stain pinealocytes as effectively.
Some cells had been immunoreactive for substance-P, oxytocin, vasopressin, leu-enkefalin and glutamic acid decarboxylase (GAD). These immnoreactivities confirmed colocalization with neuron-specific nuclear protein immunoreactivity indicating that these cells are neurons. Calbindin was noticed in oval and elongated cells resembling pinealocytes.

Dopamine Transporter (DAT) Antibody

20-abx172157
  • EUR 815.00
  • EUR 425.00
  • 1 mg
  • 200 ug
  • Please enquire.

Dopamine Transporter (DAT) Antibody

20-abx172158
  • EUR 871.00
  • EUR 453.00
  • 1 mg
  • 200 ug
  • Please enquire.

Dopamine Transporter (SLC6A3) Antibody

20-abx241713
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul
  • Shipped within 5-10 working days.

Dopamine Transporter (SLC6A3) Antibody

20-abx317926
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Dopamine Transporter (SLC6A3) Antibody

abx430757-200ul 200 ul
EUR 384
  • Shipped within 1-3 working days.

Dopamine Transporter (DAT) Antibody

abx232247-100ug 100 ug
EUR 509
  • Shipped within 5-12 working days.

anti-Dopamine Transporter

YF-PA14675 100 ug
EUR 403
Description: Rabbit polyclonal to Dopamine Transporter

anti-Dopamine Transporter

YF-PA24714 50 ul
EUR 334
Description: Mouse polyclonal to Dopamine Transporter

Dopamine Transporter (SLC6A3) Antibody (HRP)

20-abx314929
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Dopamine Transporter (SLC6A3) Antibody (FITC)

20-abx314930
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Dopamine Transporter (SLC6A3) Antibody (Biotin)

20-abx314931
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Dopamine Transporter, CT Polyclonal Antibody

ABP-PAB-22006 100 ug Ask for price
    • Product line: Pan-specific Antibodies
    • Brand:

Dopamine Transporter, EL2 Polyclonal Antibody

ABP-PAB-22007 100 ug Ask for price
    • Product line: Pan-specific Antibodies
    • Brand:

Dopamine Transporter recombinant monoclonal antibody

A5144 100ul X 3
EUR 595
  • Comparisons between Mnoclonal, Polyclonal and Recombinant antibodies and their benefits: Regular monoclonal antibodies have higher purity, better specificity and less lot-to-lot variations than polyclonal antibodies. Recombinant antibodies, however,
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Description: A recombinant monoclonal antibody from rabbit against human Dopamine Transporter for WB,ELISA

Anti-Dopamine Transporter (Thr53) antibody

STJ120056 100 µl
EUR 526

Recombinant Dopamine Transporter (DAT)

4-RPB625Hu01
  • EUR 494.24
  • EUR 235.00
  • EUR 1578.40
  • EUR 592.80
  • EUR 1085.60
  • EUR 394.00
  • EUR 3796.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: Q01959
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 39.2kDa
  • Isoelectric Point: 6.9
Description: Recombinant Human Dopamine Transporter expressed in: E.coli

Recombinant Dopamine Transporter (DAT)

4-RPB625Ra01
  • EUR 512.16
  • EUR 240.00
  • EUR 1645.60
  • EUR 615.20
  • EUR 1130.40
  • EUR 406.00
  • EUR 3964.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: P23977
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 10.5kDa
  • Isoelectric Point: 9.4
Description: Recombinant Rat Dopamine Transporter expressed in: E.coli

Polyclonal Dopamine Transporter, C-Terminus Antibody

APR11781G 0.1ml
EUR 528
Description: A polyclonal antibody raised in rabbit that recognizes and binds to Human Dopamine Transporter, C-Terminus . This antibody is tested and proven to work in the following applications:

Polyclonal Dopamine Transporter (DAT) (extracellular) Antibody

AMM05836G 0.05ml
EUR 659
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Dopamine Transporter (DAT) (extracellular) . This antibody is tested and proven to work in the following applications:

Anti-Dopamine Transporter Rabbit Monoclonal Antibody

M00369 100ug/vial
EUR 397
Description: Rabbit Monoclonal Dopamine Transporter Antibody. Validated in Flow Cytometry, IP, WB and tested in Human.

Dopamine Transporter (DAT) Polyclonal Antibody (Human)

4-PAB625Hu01
  • EUR 239.00
  • EUR 2391.00
  • EUR 598.00
  • EUR 299.00
  • EUR 211.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: DAT (Trp562~Val620)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Dopamine Transporter (DAT)

Dopamine Transporter (DAT) Polyclonal Antibody (Rat)

4-PAB625Ra01
  • EUR 251.00
  • EUR 2589.00
  • EUR 643.00
  • EUR 317.00
  • EUR 216.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: DAT (Ile562~Leu619)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rat Dopamine Transporter (DAT)

Rat Dopamine Transporter ELISA kit

E02D0140-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Rat Dopamine Transporter in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Dopamine Transporter ELISA kit

E02D0140-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Rat Dopamine Transporter in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Dopamine Transporter ELISA kit

E02D0140-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Rat Dopamine Transporter in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Dopamine Transporter ELISA kit

E01D0140-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Human Dopamine Transporter in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Dopamine Transporter ELISA kit

E01D0140-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Human Dopamine Transporter in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Dopamine Transporter ELISA kit

E01D0140-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Human Dopamine Transporter in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Primarily based on the outcomes obtained in grownup mammals, the pineal neurons could also be analogue to retinal ganglion cells, or they might perform as interneurons within the retino-pinealo-retinal neuronal circuit or the peptidergic neurons might affect the pinealocytes in a paracrine method.

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