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Co-targeting strategy for precise, scarless gene editing with CRISPR/Cas9 and donor ssODNs in Chlamydomonas

Co-targeting strategy for precise, scarless gene editing with…

Programmable site-specific nucleases, such because the clustered often interspaced brief palindromic repeat (CRISPR)/ CRISPR-associated protein 9 (Cas9) ribonucleoproteins (RNPs), have allowed creation of priceless knockout mutations and focused gene modifications in Chlamydomonas (Chlamydomonas reinhardtii). Nonetheless,…
Screening Bacterial Colonies Using X-Gal and IPTG: α-Complementation.

Screening Bacterial Colonies Using X-Gal and IPTG: α-Complementation.

Many plasmid vectors (e.g., the pUC collection, Bluescript, pGem, and their derivatives) carry a brief phase of Escherichia coli DNA containing the regulatory sequences and the coding data for the primary 146 amino acids of β-galactosidase. Vectors of this kind…